Fast sybr green master mix manual
Brilliant III Ultra-Fast SYBR Green QRT-PCR 2× Master Mix The 2× master mix contains an optimized RT-PCR buffer, MgCl 2, nucleotides (GATC), stabilizers, SYBR Green I dye and mutant Taq DNA polymerase. The DNA polymerase features a rapid hot start capability that reduces nonspecific product formation. DTT. The Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix is a single-tube reagent designed for performing accelerated quantitative PCR amplifications on the ABI StepOnePlus and Bio-Rad CFX96 real-time PCR instruments and other fast-cycling systems (such as the ABI HT and Fast systems). The master mix includes two key components that. The Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix is a single-tube reagent designed for performing accelerated quantitative PCR amplifications on the ABI StepOnePlus and Bio-Rad CFX96 real-time PCR instruments and other fast-cycling systems (such as the ABI HT and Fast systems). The master mix includes two key components that.
The QuantiFast SYBR Green PCR Kit is a ready-to-use master mix that eliminates the need for optimization of reaction and cycling conditions. Simply add primers and DNA template to the ready-to-use PCR master mix, and start the reaction. Follow the protocol in the handbook to get fast and reliable results on any real-time cycler. Power SYBR® Green PCR Master Mix: † Assays performed using the Power SYBR® Green PCR Master Mix are not supported using Fast mode; therefore Optical Well Fast Thermal Cycling Plates must be run under manual for detailed instructions on how to load and run the plate. 2X KAPA SYBR® FAST qPCR Master Mix (5 ml), to which 1 ml of 10X Illumina Primer Premix has been added. Six DNA Standards (fold serial dilutions, supplied in the kit). dilution of library DNA and optional , and serial dilutions of the same.
Fast SYBR® Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency. Brilliant III Ultra-Fast SYBR Green QRT-PCR 2× Master Mix The 2× master mix contains an optimized RT-PCR buffer, MgCl 2, nucleotides (GATC), stabilizers, SYBR Green I dye and mutant Taq DNA polymerase. The DNA polymerase features a rapid hot start capability that reduces nonspecific product formation. DTT. The Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix is formulated to provide superior specificity and sensitivity. It is supplied in a convenient 2X concentration pre-mix to perform real-time PCR using SYBR™ Green Dye. The master mix contains: • SYBR™ Green Dye • Dual-Lock™ DNA Polymerase, with a proprietary combination of two proprietary.
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